pGADT7Rec
pGADT7
pKLAC1
pPICZaA
pPICZaB
pPICZaC
pPICZA
pPICZB
pPICZC
pPIC9K
pPIC3.5K
pYES2
pYX212
原核表达载体
pET-11a
pET-15b
pET-11d
pET-17b
pET-21a(+)
pET-52b
pET-24c
pET-24a(+)
pET-39b
pET-9a
pET-28a(+)
pRSFDuet-1
pET-41a(+)
pET-21d(+)
pET-22b(+)
pET-32a(+)
pTYB11
pTYB2
pGEX-4T-1
pKK223-3
pGET-4T-2
pGEX-4T-3
pGEX-6p-2
pCold TF
pCold II
pCold III
pCOLD IV
pBAD/HisA
pBAD/Myc-His A
pTrc99A
pQE30
pQE60
pQE80
哺乳动物/真核表达载体
pcDNA3.1(-)
pCDNA3.1(+)
pcDNA3.1/Zeo(-)
pcDNA3.1/Zeo(+)
pCMV/myc/Cyto pShooter
pCMV/myc/Mito
pCMV/myc/Nuc pShooter
pCMV/myc/Nuc/GFP
pcDNA3.1/myc-His A
pEF1/myc-HisB
pcDNA3.1/myc-His B
pGene/V5-HisB
pRC/CMV
pTracer EF/V5-His A
pCEP4
pCEP4-CAT
pcDNA4.0TO/myc/his
pVAX1
pShuttle
pIE1-neo
pGlue
pOPRSVI/MCS
pRL-CMV
pCi-neo
pTRE-Tight-BI
pTRE2
pBACPak9
pIRES-neo
pNEBRX1-hygro
pTRE-Tight
pCAAGS
pEE12
pGN
piresneo3
Pires
病毒穿梭载体/腺病毒、逆转录病毒载体/RNA干扰载体
pAdTrack-CMV
pShuttle-CMV
pAdEasy-1
PLAPSN
Pbmnz
pBMN-ires-GFP
PLNCX
PLNCX2
pMSCV-HYG
pMSCV-neo
pMSCV-puro
pLEGFP-C1
pAAV-MCS
pAAV-lacZ
pAAV-IRES-hrGFP
pCMV-MCS
pAAV-RC
pHelper
pLVX-AcGFP1-N1
pLVX-DsRed-Monomer-N1
plenti6/V5-lacZ
PLP1
PLP2
PLP/VSVG
pLOX-CW-CRE
pLOX-gfp-iresTK
pMD2.G
pCMV-dR8.74
荧光定位载体
pEGFP1-N1
pEGFP1-C1
pEGFP-N3
pEGFP-C3
pYFP
pCFP
pOFP
pDsred2-N1
PDsred 2-1
pGL3-Promoter
pGL3-Basic
pGL3-Enhancer
pGL4.27/CRE
phMGFP
pIRES2-AcGFP1
pIRES2-EGFP
昆虫表达载体/病毒表达载体
pFastBac HT B
pFastBac HT A
pFastBac1
pVL1393
pMelBac B
pTriEx-4Neo
TriEx 1.1
pTrx-sumo
pAcGP67A
pAcHLT-B
pACSecG2T
植物表达载体pCAMBIA 1300, 1301, 1302,1303,1304,1305, 2300, 2301,pCAMBIA super1300,pCAMBIA super1300-GFP,
pPZP212,pPZP2121,pPZP212-GFP
pBI121,pBI121-GFP,pBI101,221
pRTL2 , pRTL2-GFP , pRTL2-RFP , pRTL2-YFP等等
农杆菌:LBA4404,EHA103,105,GV3101
原核表达质粒:
pET3a,
pET 3d,
pET 11a,
pET 12a,
pET 14b,
pET 15b,
pET 16b,
pET 17b,
pET 19b,
pET 20b,
pET 21a,b,d,
pET 22b,
pET 23a,
pET 23b,
pET 24a,b,
pET 25b,
pET 26b,
pET 27b,
pET 28a,b,
pET 29a,
pET 30a,
pET 31b,
pET 32a,
pET 35b,
pET 38b,
pET 39b,
pET 40b,
pET 41a,b
pET 42a,
pET 43.1a,b
pET 44a,
pET 49b
pET302,303
pET His,pET Dsb,pET GST,pET Trx
pQE2,
pQE9
pQE30,31,32,
pQE 40
pQE70
pQE80L
pQETirs system
pRSET-A
pRSET-B
pRSET-C
pGEX4T-1,-2,-3,5x-1,6p-1,6p-2,2tk,3c
pBV220,221,222
pTrcHisA,B,C pBAD24,34,43
pBAD HisA,B,C pPinPoint-Xa1,Xa2,Xa3
pMALc2x, p2x pBV220pGEM Ex1, pGEM7ZF(+),
pTrc99A, pTwin1, pEZZ18
pkk232-8,pkk 233-3,pACYC184,pBR322,pUC119
pTYB1,pTYB2,pTYB4,pTYB11
pBlueScript SK(+),pBlueScript SK(-)
pLLP ompA, pINIIIompA, pMBP-P ,pMBP-C,
大肠杆菌冷激质粒:
pColdI
pColdII
pColdIII
pColdTF
原核共表达质粒:pACYCduet-1,pETduet-1,pCDFduet-1,pRSFduet-1
Takara公司大肠杆菌分子伴侣:
pG-KJE8
pGro7
pKJE7
pGTf2
pTf16
大肠杆菌宿主细胞:
DH5a
JM101
JM103
JM105
JM107
JM109
JM110
Top10
Top10F
BL21(DE3)
HB101
ER2529
E2566
C2566
MG1655
XL-10gold
XL blue
M15
JF1125
K802
SG1117
BL21(AI)
BL21(DE3)plysS
TG1
TB1
DH5a(pir)
Tuner(DE3)
Bl21 codonplusRIPL
Novablue(DE3)
Rosetta
Rosetta(DE3)
Rosetta(DE3)plys
Rosetta-gami(DE3)
Rosetta-gamiB(DE3),
Rosetta-gamiB(DE3)plysS
Orgami(DE3)
OrgamiB(DE3)
HMS174(DE3)
(2)酿酒酵母表达质粒:
pYES2.0
pYIP5
pYRP7
pADH2
pYES2NT/A,B,C
pYES3
pRS426
酿酒酵母宿主菌
INVScl
(3)毕赤酵母表达质粒
pPIC9
pPIC9k
pPIC3.5
pPIC3.5K
pPICZαA,B, C
pPICZA,B,C
pGAPZαA, B,C
pAO815,pHIL-S1
毕赤酵母宿主菌:
GS115,GS190,GS200
KM71, KM71H
X33
SMD 1168, SMD1163, SMD1168H
(4) 植物表达载体
pCAMBIA 1300
pCAMBIA 1301
pCAMBIA 1302
pCAMBIA 1303
pCAMBIA 1304
pCAMBIA 1305
pCAMBIA 2300
pCAMBIA 2301
pCAMBIA 1381Z
pCAMBIA 1391Z
pCAMBIA 3300
pCAMBIA 3301
pBI 121,101,221 等等
农杆菌:LBA4404,EHA103,105
枯草芽孢杆菌表达系统:
pHCMC05
pHP13
pHP13-43
pMA5
pHT304
枯草宿主菌
BS168
WB600
1A75
巨大芽孢杆菌表达系统
Invitrogen bac to bac昆虫表达系统
真核表达质粒:
EGFP-N1,N3,C1,C2
EGFP-1, EYFP-N1,C1,
pDsRedN1,C1
pDsRed2N1,
细胞定位荧光载体
pDsRed2-ER
pDsRed1-mito
pDsred2-mito
EYFP-Mem
pECFP-N1.C1
PCDNA3.1(+)
PCDNA3.1(-)
PCDNA3.1(neo+)
PCDNA3.1(zeo+)
pCDNA3.1mychisA,
pCDNA3.1mychisB
PCDNA4HisMXHisB
pcDNA3.1(-)Hygro
pcDNA3.1(-)Zeo
pCMV-CMV
pCMV-HA
海肾荧光素酶报告基因载体
pGL3basic
pGL3control
pGL3promotor
pGL3enhancer
pGL4.26, 4.29
海肾萤光素酶对照报告基因载体:PRL-TK,PRL-CMV
其他真核载体
pVAX1
pCI
pIRES
pIRES2-EGFP
pSUPER-EGFP
pSUPER-retro-EGFP
pSUPER
腺病毒全套:
BJ5183
pADEASY-1
pADEASY-1/ BJ5183
pshuttle
pshuttle-cmv
Clontech 酵母单杂交系统
Clontech 酵母双杂交系统 pGBKT7,pGADT7,pCL1,pGBKT7-53,pGADT7-T,pGBKT7-Lam
宿主菌AH109
Clontech 酵母三杂交系统 6个质粒,一个宿主菌YBZ
Invitrogen 酵母表面展示系统 pYD1, 宿主菌EBY100
Invitrogen tet on 系统
Clonetech哺乳动物双杂交系统
噬菌体表面展示系统
Invitrogen公司酵母表面展示系统 pYD1和EBY100
基因:
人端粒酶基因
T4连接酶基因
Taq酶基因
mmlv酶基因
ung酶基因
pfu酶基因
肿瘤坏死基因
透明颤菌血红蛋白基因
酵母双杂交系统(BD公司):酿酒酵母Y187,酿酒酵母AH109;质粒pGADT7,pGBKT7;
对照质粒pGBKT7-53,pGBKT7-lam,pGADT7-T,PCL1。
酿酒酵母表达系统(invitrogen公司):质粒pYES2;宿主菌酿酒酵母INVSc1
毕赤酵母表达系统(invitrogen公司):分泌型质粒pPIC9K;pPIC3.5K 宿主菌毕赤酵母GS115
分泌型质粒pGAPZ alphaA(不需要诱导即可表达) 宿主菌毕赤酵母GS115
分泌型质粒pPICZ alpha (甲醇诱导) 宿主菌毕赤酵母x33
分泌型质粒pHIL-S1
原核表达质粒: PET28-(a,b,c) ,PET30a , PET32a,pET15,PET20b,pET29a,pET21b, pET302,pET303 ;pRSETA,pRSET C;
pBV220 ;PGEX-6P-1,pGEX4T-1,pGEX-2TK;pQE30,pQE31,pQE32,pQE40;
真核表达质粒: PVAX1,pEGFP-C1,pEGFP-N1,pIRES(neo)
克隆载体: pGEM11Zf+,pGEM-7Z,pGEM5Z,pUC19,pUC18,pBluescript II KS(+)
其他质粒: pBAD24,pBAD34,pBAD43,pSU18,ptac18,P1噬菌体,**质粒,
GFP基因,Taq酶基因 pcdna3.1_man, pRNAiT-U6_1-Neo全套,Pcmv-myc
大肠杆菌感受态:BL21,BL21(DE3),BL21(RDE3),BL21(DE3)plys(S),
JM109,DH5alpha,M15
大肠杆菌甲基化酶缺陷型感受态:JM110
植物表达载体: pBI101,121,221
pCAMBIA系列载体:pCAMBIA1300,1301,2300,2301,3300,3301
pCAMBIA1380,pMOGMON,及农杆菌转染菌株(cm抗性)
PTEN 质粒 : beta-betacatenin质粒
Vectors & strains:
哺乳动物双杂交系统(Promega):
pACT,pBIND,pACT-MyoD,pBIND-Id,pG5luc
哺乳动物双杂交系统(Stratagene):
pCMV-BD, pCMV-AD, pBD-p53, pBD-NF?κB, pAD-SV40T, pAD-TRAF,pFR-luc
酵母双杂交系统(Clontech):
pGBKT7,pGADT7,pCL1,pGBKT7-53,pGADT7-T,pGBKT7-Lam,配套酿酒酵母AH109
酵母单杂交系统(Clontech):pHis2
Cytotrap Two-Hybrid System(Stratagene):
pSos, pSos MAFB, pMyr
蜕皮激素诱导系统(Invitrogen):pIND, pVgRxR
LacSwith II哺乳动物诱导表达系统(Stratagene):pOPRSVI ,pOPI3CAT,pCMVLacI
GeneSwitch System(Invitrogen):pSwitch,
腺病毒系统(Stratagene):
pAdEasy-1,pShuttle-CMV,pShuttle,配套大肠杆菌BJ5183
腺相关病毒系统(Stratagene):
pAAV-MCS,pAAV-RC,pHelper,pAAV-LacZ,pAAV-IRES-hrGFP,pCMV-MCS
酵母表面展示系统(Invitrogen):
pYD1,配套酿酒酵母EBY100
哺乳动物表面展示系统(Invitrogen):pDisplay
四环素调控系统(Invitrogen):pcDNA4/TO/Myc-His A,pcDNA4/TO/Myc-His B,pcDNA4/TO/Myc-His C,pcDNA4/TO/Myc-His/LacZ,pcDNA6/TR
四环素调控系统(Clontech):pTet-On,pTet-Off,pTRE2,
pRevTRE,pRevTet-On,pRevTet-off
信号通路报告载体(Clontech):
pGAS-TA-Luc,pSTAT3-TA-Luc, pISRE-TA-Luc, pTA-Luc,pIκB-EGFP,
pNFAT-TA-Luc,pCaspase3-sensor,pAP1(PMA)-Luc
信号通路报告载体(Promega):
pGL4.26[luc2P/minP/Hygro],pGL4.29[luc2P/CRE/Hygro],pGL4.30[luc2P/NFAT-RE/Hygro],pGL4.75
信号通路报告载体(Stratagene):
p53-Luc,pAP-1-Luc, pNF-κB-Luc,pSRE-Luc,
pFA2-Elk1,pFC-MEKK,pFR-luc
Gateway系统(invitrogen):
pcDNA6.2-GWEmGFP-miR negative, pLenti 6/TR
pcDNA 6.2-GW EmGFP-miR ,
逆病毒载体(Clontech):pLNCX2,pQCXIH,pMSCVpuro
逆病毒载体(addgene ):pLVTH
慢病毒载体(addgene): FUGW,pCMV-dR8.91,pCMV-VSV-G
(addgene):pRSV-rev,pLentilox 3.7,pMDLg pRRE (pCMV-VSV-G)
慢病毒载体(addgene):pWPXL
慢病毒载体(clontech):pLVX-DsRed-Monomer-N1
慢病毒包装载体(invitrogen): pLP1, pLP2. pLP VSV-G,
慢病毒包装载体 (clontech): pVSV-G
RNAi载体(Ambion):
pSilencer1.0,pSilencer 2.1-U6 hygro,
pSilencer 3.1-H1 hygro,pSilencer 3.1-H1 neo,
pSilencer 4.1-CMV neo,
pSilencer 4.1-CMV puro(HindIII-BamHI:2000bp插入序列),
RNAi载体(clontech):
RNAi-Ready pSIREN-Retro Q,
RNAi-Ready pSIREN-RetroQ-ZsGreen(Luciferase shRNA Annealed Oligonucleotide)
国产:pGenesil-1
酵母表达载体(Invitrogen):
pGAPZα A,pPICZα A,配套毕赤酵母X33,KM71,KM71H,GS115,SMD1168,SMD1163,
酵母表达载体(Invitrogen):pYES2,pYES6/CT,配套酿酒酵母INVSc1
哺乳动物表达载体(Invitrogen):
pcDNA3.1(neo)+,pcDNA3.1(neo)-,pcDNA3.1(hyg)+,pcDNA3.1(zeo)+,pcDNA3.1/GS,pcDNA4/HisMax B,pSecTag2 A,pVAX1,pBudCE4.1,
pTracer CMV2,pcDNA3.1(-)/myc-His A ,pcDNA6-Myc/His B, pCEP4(附加体形式), pMIB v5-His B,pGene v5-His B,pUB6/V5 His A
(Stratagene): pCMV-Tag2B,
(Promega)pCI-neo,pCI,pSI,
(Clontech)pIRES,pIRESneo,pIRES hyg3,pCMV-myc,pCMV-HA,
重组蛋白乳汁表达系统(Invitrogen):pBC1
荧光蛋白报告载体(Clontech):
pDsRed1-C1, pDsRed1-N1, pDsRED-Monomer-N1, pEYFP-C1, pEYFP-N1, pEGFP-1,pEGFP-C1, pEGFP-N1, pEGFP-N3, pcDNA-EGFP1(实验室构建:EcoRI-HindIII), pECFP-N1,pECFP-C1, pBridge,pAcGFP1-1
pDsRED2-ER, pDsRED2-Mito, pDsRED2-Nuc,pDsRED2-Peroxi ,
pEYFP-Golgi, pEYFP-Mem, pEYFP-ER , pEGFP-Actin, pECFP-ER,
pIRES2-EGFP, pLEGFP-C1,pLEGFP-N1,pmCherry-N1,pDsRED-Express-N1
启动子增强子报告载体(Promega):
pGL3-basic,pGL3-control,pGL3-enhancer,pGL3-promoter,pRL-TK,pRL-CMV,pRL-SV40
昆虫细胞表达载体(Invitrogen):
pFastBac1,pFastBac1-Gus,pFastBacHT A,pFastBacHT-CAT,pFastBacDual,pBlueBacHis2 A,配套大肠杆菌DH10Bac
pIZT/V5-His
原核表达载体(Novagen):
pACYCDuet-1, pET-11a(+), pET-17b(+), pET-19b(+), pET-20b(+),pET-22b(+), pET26b+,pET-27b(+), pET-28a(+), pET-32a(+),pET-39b(+),pET-40b(+),pET-42a(+),pETduet-1,pRSFDuet- 1,pET50b(+)
配套大肠杆菌(Novagen):
BL21, BL21(DE3), BL21(DE3)plysS, Rosetta-gami B(DE3)pLysS,BLR(DE3)
原核表达载体(NEB):
pTWIN1,pTYB11,pMXB10,pTXB1配套大肠杆菌ER2566
原核表达载体(Amersham):pGEX-6P-1,
基因载体:(Invitrogen)pOG44
(Invivogen):pORF-lacZ,pORF-HSV1tk
克隆载体:(Promega):pSP73,pUC18,pBR322,pSP64
Cosmid vector (Stratagene): SuperCos 1
克隆菌株:JM109,JM110,TOP10,XL1-Blue,MEDH5α,SEDH5α,
XL2-Blue MRF’,Mach1 T1,
Genes:
HPV16 E5,HPV16 E6,HPV16 E7,human CD40L,human DHFR,human Hspa1a
human IgG1 Fc,human H-Ras(activated),human TERT,human EGF, mouse GM-CSF,mouse IFN-gamma,mouse IL4,mouse IL18,
Taq DNA polymerase, SV40-Large T(pREV TRE)
另外可以提供其他各种种类载体和菌株,并提供代做实验如质粒构建、蛋白原核、真核表达及纯化等服务。